Page 21 - pest-POSTEN nr 4,1996

Side 21 -pest-POSTEN

we could not observe any significant changes in depressed in all HIV patients. Both IL-12 and
Fas-ag expression on PBMC after IVIG infusion, anti-IL 10 induced a significant increase in
there was a marked and rapid ( 1 h) increase in proliferation (1,5-2,3 fold) in asympt. patients,
levels of sFas-ag in plasma which persisted while no significant response was seen in the
elevated throughout the study. 4. There was a AIDS patients. IL-10 production in response to
reduction in both unstimulated and LPS stimulated MAC antigens was significantly higher in HIV
apoptosis in PBMC in vitro after IVIG patients than in the controls, the highest IL-10
administration in vivo. 5. There was a significant levels were found in cultures from the AIDS
increase (approx. 40%) in CD4+ lymphocyte patients. In conclusion, the high production of
counts in peripheral blood 140 h after IVIG IL-10 and lack of proliferative response to IL-12
administration. Thus, IVM infusion may after MAC antigen stimulation may be of
downregulate abnormally increased TNF activity importance in the immunopathogenesis of MAC
and possibly "neutralize" Fas activation by infection in AIDS patients. Compared to the AIDS
increasing levels of sFas-ag, and may represent a patients, asymptomatic patients respond to MAC
potential immunmodulating therapeutic agent in antigens with lower IL-10 production and a
HIV infection. significant proliferative response to IL-12.

INTERLEUKIN (IL)-12 AND ANTI-IL-10 LOW EXPRESSION OF TNF-p75
STIMULATE LYMPHOCYTE RECEPTOR ON MONONUCLEAR CELLS
PROLIFERATION TO MYCOBACTERIUM IS ASSOCIATED WITH ADVANCED
AVIUM ANTIGENS IN PATIENTS WITH IMMUNODEFICIENCY AND HIGH VIRAL
ASYMPTOMATIC HIV INFECTION, BUT LOAD IN HIV INFECTION
NOT IN AIDS PATIENTS Müller F, Hestdal K, Aukrust P, Lien E, Espevik T,
Müller F, Aukrust P,. Haug CJ, Frøland SS. Frøland SS. Section of Clin. Immunology and
Section of Clin. Immunology and Infectious Infectious Diseases, Med. Dept. A, and Dept. of
Diseases, Med. Dept. A and Res. Institute for Pediatric Research Univ. of Oslo, Rikshospitalet,
Internal Medicine, University of Oslo, Oslo, and Inst. of Cancer Research Univ. of
Rikshospitalet, N-0027 Oslo, Norway. Trondheim, Trondheim, Norway.

Disseminated Mycobacterium avium complex TNF and its two receptors, pS5 and p75, may
(MAC) infection is one of the most frequent play an important role in the immunopathogenesis
infections in AIDS patients. Altered regulation of of HIV infection. Several reports have shown
cytokines like IL-10 and IL-12 is probably of elevated plasma levels of soluble TNF, p55, and
importance in the immunopathogenesis of MAC p75, while little is known about the expression of
infection in AIDS. The purpose of the study was to the corresponding membrane bound forms. In this
examine the effect of IL-12 and neutralizing study, we compared the expression of TNF, p55
anti-IL-10 on in vitro proliferative responses to and p75 on mononuclear cells Mom patients with
different MAC antigens of peripheral blood different clinical stages of HIV infection to plasma
mononuclear cells from HIV-infected patients and levels of circulating TNF, p55, p75, and HIV
to assess cytokine production in MAC antigen RNA. Expression of TNF, p55, and p75 was
stimulated cell cultures from the same patients. examined by flow cytometry on mononuclear cells
Lymphocyte proliferation from 15 patients with (MNC) Mom 12, 11, and 10 HIV-seropositive
asymptomatic HIV infection and 9 patients with patients in CDC (Centers for Disease Control)
AIDS was assessed after stimulation with two group A, B. and C, respectively. Also, 12 controls
different MAC antigens. Mononuclear cells from were studied. Compared to controls, MNC from
patients and controls (n=21) were cultured with or patients in CDC group A and B had significantly
without anti IL-10 or IL-12 for 6 days and higher expression of TNF, p55, and p75. In
proliferation quantified by incorporation of contrast, MNC from AIDS patients (CDC C)
3H-thymidine. IL-10 was quantified by ELISA in expressed significantly lower levels of TNF
culture supernatants. MAC-antigen stimulated p55, and p75 than the other HIV patients. The
lymphocyte proliferation was significantly